Extended Data Fig. 2: Cell clustering and annotation.
From: Time-resolved assessment of single-cell protein secretion by sequencing
(A–C) Unsupervised cell clustering based on the expression of a panel of surface protein markers as listed in B. The ‘negative population’ (blue arrow) was determined as the cluster with the lowest specific protein expression. Shown are UMAP plots for all cells from integrated time points (A) as well as for cells from individual time points (C). (D) Manual cell type annotation based on the expression profile of surface protein markers and a panel of genes relating to immune cell activation/differentiation. (E) Cells from all time points were integrated and clustered by their gene expression profiles (left) and colored by cell subtypes (right) as annotated in D.
