Extended Data Fig. 9: Performance of NEMO in visual cortex neurons from mouse and leaves of Arabidopsis thaliana.
From: Engineering of NEMO as calcium indicators with large dynamics and high sensitivity
(a) Typical two-photon images of NEMOs-expressing neurons obtained from the visual cortex of mice. 920-nm light was used to excite NEMOs. (b) Comparison of the basal fluorescence of NEMOs, NEMOf and GCaMP6s under different depth beneath the surface of mouse brain. 920-nm light was used (n = 8 ROIs from 2 mice for per sensor). (c) More than two months after infection, the basal fluorescence of NEMOm of the same set of cells excited by light at different wavelengths was quantified (n = 20 cells). Data in (B) and (C) were shown as mean ± s.e.m. (d) Cumulative distribution of SBR (left) or SNR (right) of NEMOf excited at 920-nm (n = 40 cells) or 980 nm (n = 22 cells). GCaMP6f and NEMOf data were replotted from Extended Data Fig. 8g. (Left, NEMOf vs NEMOf-980, p = 0.95; Right, GCaMP6f vs NEMOf-980, ***, p = 0.0005; NEMOf vs NEMOf-980, **, p = 0.008; Kolmogorov-Smirnov test, two-tailed). e) Ca2+ oscillations near plasmodesmata in the leaves of Arabidopsis thaliana when excited at 488 nm. NEMOm was tagged with PDLP1, a plasmodesmata marker. Scale bar, 5 μm. Left, cartoon illustration showing the structure of plasmodesmata; right, typical traces. At least n = 3 independent biological replicates.
