Extended Data Fig. 8: PE-tag in HEK293T cells.
From: Genome-wide profiling of prime editor off-target sites in vitro and in vivo using PE-tag
a and b, Subset of potential off-target (OT) sites identified by PE-tag using PE2 RNP or expression plasmid treated cells at VEGFA locus (a) and CDH4 locus (b; Supplementary Data 1). Mismatches in the PBS and HA region for potential off-target sites relative to the target site (On) are shown in red and blue, respectively. UMI counts for each site are shown for each treatment. c, Indel rates for tag insertion pegRNAs with PE2 at top 5 potential off-target sites identified by in vitro PE-tag for VEGFA locus pegRNA in HEK293T cells. Indel frequencies were quantified by deep sequencing from PCR amplicons spanning each locus. Results were obtained from three independent experiments and presented as mean ± SD. ** P < 0.01 and *** P < 0.001 by unpaired, two-tailed Student’s t-test. To adjust for multiple comparisons, p-values were adjusted using the Benjamini-Hochberg (BH) method.
