Extended Data Fig. 9: Prime editing at a subset of off-target sites for three pathogenic correcting pegRNAs.
From: Genome-wide profiling of prime editor off-target sites in vitro and in vivo using PE-tag
a, Venn diagram of overlap between potential off-target sites (UMI > 1) discovered by in vitro PE-tag and potential off-target sites discovered by GUIDE-tag in cell lines containing the pathogenic sequences treated with SpCas9 RNP and DSB tagging oligonucleotide. b-c, Comparison of editing rates by prime editor programmed with pegRNA to correct pathogenic sequence at a subset of potential OT sites identified by PE-tag in cells transfected with PE2 mRNA and pegRNA, plasmids expressing PE2 and pegRNA, or plasmids expressing PE2 and epegRNA at the CFTR locus (b) and MECP2 locus (c), respectively. Frequencies of editing rates were quantified by deep sequencing. Results were obtained from three independent experiments and presented as mean ± SD. *P < 0.05, ** P < 0.01 and *** P < 0.001 by unpaired, two-tailed Student’s t-test. To adjust for multiple comparisons, p-values were adjusted using the Benjamini-Hochberg (BH) method.
