Extended Data Fig. 3: The prime editing efficiency of 3′ flap generation with a series of pegRNAs.
From: Genome-wide profiling of prime editor off-target sites in vitro and in vivo using PE-tag
a, The prime editing efficiency of 3′ flap generation with a series of pegRNAs which contain either one or two mismatches in the PBS region. HEK293T gDNA was treated with PE2 RNP containing the HEK4 20-7 pegRNA to introduce the 3′ flap for 2 hours, and then the flap incorporation efficiency was quantified by qRT-PCR with a tag-specific primer and a locus-specific primer. A pair of primers located ~2000 bp upstream of the target site serve as an internal control for data analysis. b, The efficiency of 3′ flap generation at HEK OT3 with a series of HEK4 20-7 pegRNAs which contain either one or two mismatches in the PBS region. HEK293T gDNA was treated with PE2 RNP to introduce the 3′ flap, and then the editing efficiency was quantified by qRT-PCR with a tag-specific primer and a locus-specific primer. A pair of primers located ~2000 bp upstream of the target site serve as an internal control for data analysis. Where shown, bar charts indicate the mean and error bars are s.d. of n = 3 independent qRT-PCR experiments.
