Extended Data Fig. 5: Glutamate titrations. | Nature Methods

Extended Data Fig. 5: Glutamate titrations.

From: Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission

Extended Data Fig. 5: Glutamate titrations.The alternative text for this image may have been generated using AI.

a) Titrations of WT (SF-Venus-iGluSnFR-A184V), iGluSnFR3.v82 and iGluSnFR3.v857 on the surface of cultured hippocampal neurons. Baseline, 100 mM glutamate, and ∆F/F0 heat maps shown. b) ∆F/F0 affinity curves. ‘APO’ represents brightness in imaging buffer without added glutamate. ∆F/F0 is computed as fractional change in intensity of entire field of view (FOV) relative to corresponding APO image. The points are fit using a sigmoidal curve.

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