Extended Data Fig. 6: Breeding for SELECTIV-KO-Myh6 mice with heart cardiomyocyte specific AAVR overexpression.
From: Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression
SELECTIV (LSL-Aavr-mCh+/+, Aavr+/−) male and SELECTIV-KO (LSL-Aavr-mCh+/+, Aavr−/−) female mice were crossed to generate and maintain SELECTIV-KO female mice for breeding. Male mice on the BL/6 background that are Aavr−/− are sterile, so they must be maintained as Aavr+/−. Myh6-Cre (Myh6-Cre+/+, Aavr+/+) male mice were purchased from Jax (Stock No: 011038) and bred with AAVR-KO (Aavr−/−) female mice to generate Myh6-Het (Myh6-Cre+/−, Aavr+/−) male mice (optional breeding can be carried out to generate Myh6-Cre+/+, Aavr−/− males). The F1 generation SELECTIV-KO and Myh6-Het mice were bred to generate the F2 mice, which results in SELECTIV (LSL-AAVR+/−, Myh6-Cre−/−, AAVR+/−), SELECTIV-KO (LSL-Aavr-mCh+/−, Myh6-Cre−/−, Aavr−/−), SELECTIV-KO-Myh6 (LSL-AAVR-mCh+/−, Myh6-Cre+/−, Aavr−/−), and SELECTIV-WT-Myh6 (LSL-Aavr-mCh+/−, Myh6-Cre+/−, Aavr+/−). The SELECTIV-KO-Myh6 mice are used to show specific targeting of the heart with detargeting in the rest of the body, while other mice can be used as controls.
