Extended Data Fig. 8: Transduction of SELELCTIV-KO-Alb mice.
From: Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression
a, In vivo imaging of mice with liver specific expression of AAVR transduced with AAV9-luc. Control mice (Aavr+/−) or SELECTIV-KO-Alb mice were injected intravenously with AAV9 encoding luciferase. Representative mice are shown for mice on days 7, 14, and 20 mice after injection with luciferin and imaging using the Lago in vivo imaging system. Luciferase activity can be seen throughout the body of Control mice, while high levels of activity can only be seen in the abdomen of SELECTIV-KO-Alb mice. b, Specific transduction of SELECTIV-KO-Alb mouse livers by AAV-GFP. Control and SELECTIV-KO-Alb mice were transduced with AAV-PHP.eB encoding EGFP. 28-days post AAV transduction, hearts and livers were removed, and transduction was determined by microscopy (GFP; green). Hoechst nuclear staining in blue. Stitched pictures are shown for the inlays (dotted boxes) shown at higher magnification in Fig. 5c. Scale bar: 1 mm.
