Extended Data Fig. 1: Breeding for SELECTIV-WB full body AAVR-overexpressing mice.
From: Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression
SELECTIV (LSL-Aavr-mCh+/+, Aavr+/+) mice were bred with E2A-Cre mice from the Jackson Laboratory (E2A-Cre+/+ Aavr+/+), which express Cre in early embryogenesis. This results in the F1 generation, which had partial editing of the LSL allele and results in mosaic overexpression of the Aavr-mCh transgene, including in some germline cells. These mice are crossed with wild-type mice to generate the F2 generation, where some mice will be Aavr-mCh+/− and E2A-Cre−/− (SELECTIV-WB). These mice are then crossed to generate the F3 generation, which includes the SELECTIV-WB mice (Aavr-mCh+/+, Aavr+/+), which were maintained as a pure line of AAVR-mCh overexpressing mice.
