Extended Data Fig. 2: AAVR protein expression and immune cell profiling in mouse lines.
From: Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression
Tissue from Aavr+/+, Aavr+/−, Aavr−/− and, SELECTIV-WB mice were homogenized and AAVR and GAPDH protein was detected by western blotting. Samples were normalized for tissue weight and equal amounts were loaded except for the SELECTIV-WB samples for heart and lung, which were diluted 1:10 prior to loading. AAVR was not detected in AAVR-KO mice, while SELECTIV-WB mice have highly increased AAVR protein levels. b, Semi-quantitative analysis was performed by quantifying the relative expression of AAVR compared to GAPDH for each sample (all samples normalized to Aavr+/+ for each tissue). Mean value and SEM are shown (n = 3 mice) c-d, Immune cells populations in the spleen (c) and circulation (d) were quantified. There were no apparent differences in cell quantities compared to Aavr+/+ mice.
