Extended Data Fig. 5: Responses of ASAP family GEVIs to AP waveforms in HEK293A cells.
From: A positively tuned voltage indicator for extended electrical recordings in the brain
(A) Responses of indicators in HEK293a cells to an AP waveform (far left) under voltage clamp that has been modified to have either a 2 ms FWHM (top), or a 4 ms FWHM (bottom), and ranges from -70mV to +30 mV. Each cell had the waveform applied 5 times, with n = 6 cells for ASAP3, n = 7 cells for ASAP4b, n = 5 for ASAP4e, and n = 7 cells for Upward Ace2mNeon. Shading is SEM. (B) Response to commanded spike trains in HEK293A cells at room temp. Vm was –70 mV at the start of the displayed segment and peak AP amplitude was 100 mV. Responses were collected from 6 (ASAP4.2), 7 (ASAP4b), 5(ASAP4e), 6 (ASAP3) and 7 (Upward Ace2mNeon) HEK293A cells, each supplying a single unfiltered and unsmoothed 1000-Hz trace. Colored lines represent averages, gray shading is SEM. (C) Responses of indicators in HEK293A cells an AP burst waveforms of 1–4 spikes with spike widths of 1.0 ms at FWHM. These waveforms were previously recorded from CA1 mouse hippocampal pyramidal neurons in vivo. Vm was –52 mV at the start of the displayed voltage segment. Each colored line is a single unfiltered trace with no trial averaging or filtering applied.
