Extended Data Fig. 10: In vivo voltage imaging under one-photon wide-field illumination in hippocampus and primary motor cortex.

(A) Photostability during continuous one-photon illumination of ASAP3-Kv, ASAP4b-Kv, and ASAP4e-Kv in hippocampal SST+ interneurons in the mouse brain 4–7 weeks post-infection. Powers used were 100–250 mW/mm², and time coordinates were adjusted to simulate 100 mW/mm² assuming linearity between power and photobleaching rate. (B) A hippocampal CaMK2α + neuron expressing ASAP4b-Kv 4 weeks post-infection, imaged under one-photon illumination (150 mW/mm²) and its fluorescence trace for 30 seconds. The signals were acquired at 1000-fps and time-binned to 500fps. The trace was normalized to the mean signal of the first 100 frames and not corrected for photobleaching. The right-most zoomed-in trace shows spontaneous spikes.