Extended Data Fig. 10: Meso-scale tissue context.
From: Dense 4D nanoscale reconstruction of living brain tissue
a, Meso-scale overview volume acquired in confocal mode with select subvolume acquired and reconstructed using LIONESS in an organotypic hippocampal slice culture. A glial cell is 3D-rendered together with example neuronal processes. b, Three orthogonal planes from the automated segmentation of the LIONESS volume in panel a. Segmentation (color) and LIONESS data are overlaid. No proofreading was applied. White arrowheads at image edges indicate corresponding orthogonal planes. The same glial cell is indicated in both panels. Scale bar: 2 µm.
