Extended Data Fig. 2: Live-tissue compatibility.
From: Dense 4D nanoscale reconstruction of living brain tissue
a, Confocal overview images in an organotypic hippocampal slice culture before (left) and 10 minutes after (right) scanning a volume in the center (5 × 5 × 2.5 µm3) 20 times in high-photon load STED mode (70 µs voxel integration time). Central images are single planes of the first and last STED volume acquired. Red arrows indicate blebbing and disintegrating cells. b, Confocal overview images of a different region before (left) and 10 minutes after (right) scanning a volume in the center (5 × 5 × 2.5 µm3) 20 times using LIONESS parameters (10 µs voxel integration time). Central images are single planes of the first and last LIONESS volume acquired. The images are representative of n = 4 experiments recorded across two biological samples. Scale bars: confocal: 10 µm, STED: 1 µm.
