Extended Data Fig. 9: Conservation and spatial visualization of enhancer-GRNs in the mammalian brain.
From: SCENIC+: single-cell multiomic inference of enhancers and gene regulatory networks
a. Heat map showing the scaled correlation between the RSS values for each regulon in each cell type. b. Human cortex UMAP (84,159) showing TF expression (red) and AUC enrichment of the mouse regulon (converted to human genes). c. Barplot showing the number of conserved genes between the matching human and mouse regulons. d. Barplot showing the number of conserved regulons between the matching mouse and human regulons. e. Mapping of cell types in the mouse cortex into our smFISH map using Tangram. f. Visualization of regulons AUC enrichment in our smFISH map of the mouse cortex. g. Representative layer-specific gene regulatory network. The network depicted from L2/3 to L6 corresponds to excitatory neurons, while in the white matter corresponds to oligodendrocytes. h. SCENIC+ UMAP containing 1,736 cells from the human cerebellum. i. Human cerebellum 10x Visium slide annotated with anatomical regions in the cerebellum. j. Visualization of regulons AUC enrichment on the 10x Visium data. AST: Astrocytes, BG: Bergman Glia, CGE: Caudal Ganglionic Eminescence, ENDO: Endothelial cells, GC: Granule Cell, GP: Granule cell Progenitor, MGE: Medial Ganglionic Eminescence, MGL: Microglia, MG: Muller Glia, OL: Oligodendrocyte, OPC: Oligodendrocyte Precursor Cells, PURK: Purkinje cells, WM: White Matter.
