Extended Data Fig. 7: Cell survival after Cas9 editing for indel generation without a DNA donor and precise editing with HDRobust.
From: Efficient high-precision homology-directed repair-dependent genome editing by HDRobust
Cell survival after Cas9 edits of thirty targets in 409B2 hiPSCs without DNA donors (related sequencing data Extended Data Fig. 6) (a), and with both DNA donors and HDRobust (related sequencing data Fig. 2d) (b). Cell survival was quantified by a fluorescence resazurin assay with respect to mock electroporation without editing. Independent biological replicates are depicted by dots (n = 3) and error bars show the s.e.m. Each dot in the right panels indicate the mean of one target, the box the 25th to 75th percentile, the line the median and whiskers extend to the minimum and maximum values.
