Extended Data Fig. 8: Repeated editing using HDRobust to enrich for HDR edited cells.
From: Efficient high-precision homology-directed repair-dependent genome editing by HDRobust
(a) Genome editing efficiencies using repeated Cas9D10A double nicking or Cas9-HiFi in combination with HDRobust in H9 hESCs that carry no repair gene mutation. Frequencies of deletions are presented based on microhomology (MH) length. For HDR, replicates are depicted by dots. The mean outcome purity given below is the percentage of HDR of all editing events. (b) Cell survival corresponding to edits from panel a. Cell survival was quantified by a fluorescence resazurin assay with respect to editing in wild type cells. Independent biological replicates were performed (n = 3) and error bars show the s.e.m.
