Extended Data Fig. 3: Indel efficiencies and cell survival after editing without DNA donors.
From: Efficient high-precision homology-directed repair-dependent genome editing by HDRobust
(a) Genome editing efficiencies using Cas9D10A double nicking without a DNA donor in H9 hESCs that carry either no repair gene mutation or combinations of DNA-PKcs K3753R, Polϴ V896*, and RAD52 K152A/R153A/R156A (K/R152-156A). Frequencies of deletions are presented based on microhomology (MH) length. For insertions, replicates are depicted by dots. (b) Deletion pattern shapes of editing from panel a. The lines correspond to wild type (black) or repair gene mutants (colored). Each line is the mean of replicates. (c) Cell survival corresponding to edits from panel a. Cell survival was quantified by a fluorescence resazurin assay with respect to editing in wild type cells. Independent biological replicates were performed (n = 3) and error bars show the s.e.m.
