Extended Data Fig. 5: IMC of markers detected in breast cancer samples by MEZ-XRF.
From: Multielement Z-tag imaging by X-ray fluorescence microscopy for next-generation multiplex imaging
a, The antibody panel used to label the indicated molecular features in breast cancer samples from Fig. 4 with additional tag isotope information. b-h, IMC images of the indicated markers in breast cancer samples. Samples are presented in columns and element/marker channels in rows. The fields of view are the same as those imaged in Fig. 3 by MEZ-XRF, here images obtained subsequently by IMC are presented. Samples were scanned once with a 500-nm step (pixel size) raster at 200 Hz. Overviews and ROIs were manually selected from complete scans to match the regions shown in Fig. 3. Overview scans were down sampled 1:4 to match the 2-µm step sampling used for MEZ-XRF overviews. Images are 0.5–99.5% grey levels of IMC counts per isotope matched across samples as shown in colour bars.
