Extended Data Fig. 7: IMC images of standard and SABERx2 Z-tags in cell pellets. | Nature Methods

Extended Data Fig. 7: IMC images of standard and SABERx2 Z-tags in cell pellets.

From: Multielement Z-tag imaging by X-ray fluorescence microscopy for next-generation multiplex imaging

Extended Data Fig. 7

a, Standard conjugation panel. b, SABERx2 amplified conjugation panel. c and d, Matched IMC imaging of four epithelial cell lines (columns) labelled with c) standard Z-tags or d) SABERx2 amplified Z-tags. Top left label indicates isotope/marker (as in Extended Data Fig. 5). Bottom left indicates whether SABERx2 was used. IMC images were scanned once after MEZ-XRF using a 500-nm step (pixel size) with raster ablation at 200 Hz. These are the same sections as imaged by MEZ-XRF in Fig. 4.

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