Extended Data Fig. 10: MEZ-XRF imaging of SABERx2 Z-tags in breast tumor tissue.
From: Multielement Z-tag imaging by X-ray fluorescence microscopy for next-generation multiplex imaging
a, Antibody panel used for SABER Z-tags. b-j, MEZ-XRF imaging of breast tumour tissues stained with SABER-Z tags. Columns indicate the different tissue samples, and rows indicate markers and element/emission lines. All XRF emissions were recorded with state-of-the-art GeCMOS2. Scans were imaged with a 500-nm focused 69-keV X-ray beam with 500-nm raster steps at 50 Hz. Top left label indicate element_emission lines/marker (as in Fig. 2). Bottom left indicates whether SABERx1 or SABERx2 was used (a decision based on the expected marker amplification level given our experience with these reagents). k-s, IMC of the sections imaged by MEZ-XRF. Top left label indicates isotope/marker. Bottom left indicates whether SABERx1 or SABERx2 was used (a decision based on the expected marker amplification level given our experience with these reagents).
