Fig. 4: SABER Z-tags increase speed and sensitivity of MEZ-XRF.
From: Multielement Z-tag imaging by X-ray fluorescence microscopy for next-generation multiplex imaging
a, Schematic of SABER Z-tag element amplification. b–f, Standard MEZ-XRF imaging of the indicated markers in MCF10a, A431, ZR-75-1 and SKBR3 cell pellets (columns): I_Ka|IdU (b); Nd_Ka|HER2 (c); Dy_Ka|CK7 (d); Tb_Ka|CK5 (e) and Tm_Ka|Vim (f). Scans were imaged with a 500 nm focused 69 keV X-ray beam with 500 nm raster steps at 10 Hz. g–k, SABERx2-amplified MEZ-XRF imaging of the indicated markers in MCF10a, A431, ZR-75-1 and SKBR3 cell pellets: I_Ka|IdU (g); Gd_Ka|HER2 SABERx2 (h); Dy_Ka|CK7 SABERx2 (i); Ho_Ka|CK5 SABERx2 (j) and Lu_Ka|Vim SABERx2 (k). Scans were imaged with a 500 nm focused 69 keV X-ray beam with 500 nm raster steps at 10 Hz. l, Signal-to-noise comparison between standard MEZ-XRF and IMC. IMC images are shown in Extended Data Fig. 7a. m, Signal-to-noise comparison between SABERx2 MEZ-XRF and IMC. IMC images are shown in Extended Data Fig. 7b. n–q, High-speed (1,500 Hz) SABERx2-amplified MEZ-XRF imaging of the indicated markers in MCF10a, A431, ZR-75-1 and SKBR3 cell pellets: Gd_Ka|HER2 (n); Dy_Ka|CK7 SABERx2 (o); Ho_Ka|CK5 SABERx2 (p) and Lu_Ka|Vim SABERx2 (q).
