Extended Data Fig. 9: Control and tracking of secretory protein trafficking.
a Schematic illustrating how FIREmate-KDEL acts as a hook to retain FIREtag-ged reporter in the endoplasmic reticulum in presence of the match. Release is induced by washing of the match, allowing trafficking of the reporter to its acceptor compartment. b,c HeLa cells co-expressing FIREmate-KDEL and mApple-FIREtag-GPI were treated with match550 for 24 h and image by spinning-disk microscopy after washout of match550. b Representative micrographs before washout, 20 min and 70 min after washout (see also Supplementary Video 20). Experiments were repeated five times with similar results. c Temporal evolution of the CATCHFIRE signal in the ER (green) and the mCherry signal in the Golgi apparatus (red). Data represents the mean ± SD of 14 cells. Red: ex/em 561/604-664 nm; green: ex/em 488/545-575 nm. Scale bars are 10 μm. All images were enhanced by 2D-deconvolution on NIS-Elements Ver.5.42.
