Extended Data Fig. 4: Photostability of different FRs and FP in single-photon excitation. | Nature Methods

Extended Data Fig. 4: Photostability of different FRs and FP in single-photon excitation.

From: Large Stokes shift fluorescent RNAs for dual-emission fluorescence and bioluminescence imaging in live cells

Extended Data Fig. 4

(a) HEK293T cells expressing tRNA-Clivia or tRNA-Chili were labeled with 0.5 μM NBSI and its analogs or 1 μM DMHBI-lmi. Continuous fluorescence imaging was performed using a spinning disk confocal laser scanning microscope with a 488 nm single-photon excitation. Cells expressing CyOFP1-H2B were used as the controls. Scale bars, 10 μm. (b) Quantification of the fluorescence in (a). Data were normalized to the initial image intensity (at time 0), N=10 cells.

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