Fig. 6: Denoising voltage imaging data with motion.
From: Statistically unbiased prediction enables accurate denoising of voltage imaging data
a, Representative frames of raw video and SUPPORT-denoised videos without and with motion after baseline correction. Motion was synthetically applied to the images of neurons in mouse cortex L2/3 expressing QuasAr6a, simultaneously recorded with electrophysiology. Scale bars, 5 μm. b, Representative frames of a spatially expanded view of cell 1 in a at the timings indicated by red arrows in c. From left to right: frames at 604, 955, 2,214 and 3,521 ms. From top to bottom: raw video, SUPPORT-denoised video without motion and SUPPORT-denoised video with motion. Scale bar, 5 μm. c, Line plot showing the x and y direction motions in the micrometer scale. d, Electrophysiology trace and single-pixel fluorescence traces extracted from the videos. From top to bottom: electrophysiology, raw video, SUPPORT-denoised video without motion and SUPPORT-denoised video with motion. Scale bar, 500 ms. e, Box-and-whisker plot showing Pearson correlation coefficients between fluorescence traces and electrophysiology, before and after denoising. ×5 indicates a five times higher motion compared to ×1. n = 5, which represents the number of cells. f, Box-and-whisker plot showing Pearson correlation coefficients between ground-truth image (SUPPORT-denoised image without motion) and images with motion before and after denoising. n = 5, which represents the number of cells. g, Box-and-whisker plot showing SNR acquired by comparing ground-truth image and images with motion before and after denoising. n = 5, which represents the number of cells. h, Representative frames of raw video and SUPPORT-denoised videos after baseline correction. The images show a neuron expressing SomArchon in the hippocampus of an awake mouse. Scale bar, 3 μm. i, Representative frames in h at the timings indicated by red arrows in j. From left to right: frames at 2,018, 17,203, 29,618 and 50,025 ms. Scale bar, 5 μm. j, Line plot showing x and y directional motions in the micrometer scale. k, Traces extracted from a single cell in raw video and SUPPORT-denoised video. Temporally expanded traces from the brown area on the left are shown on the right. l, Histogram of SNR from the raw video and SUPPORT-denoised video.
