Extended Data Fig. 5: Complete GCN4 uORF1 and uORF2 and CPA1 uORF profiles for monosome, sub-disome, and true disome footprints with and without HTS1 knockdown.

a, Extended representation from Fig. 5c. Rescaled counts of 5′ and 3′ ends of aligned true disome (top), sub-disome (middle), and monosome (bottom) footprints for GCN4 uORF1 without HTS1 knockdown. Results from replicates were summed together from sucrose density gradient purified material. b, Footprint length 5′ and 3′ profile of (a). c, Rescaled counts of 5′ and 3′ ends of aligned true disome (top), sub-disome (middle), and monosome (bottom) footprints for GCN4 uORF1 after HTS1 knockdown. Results from replicates were summed together. d, Footprint length 5′ and 3′ profile of (c). e, Extended representation from Fig. 5d. Rescaled counts of 5′ and 3′ ends of aligned true disome (top), sub-disome (middle), and monosome (bottom) footprints for GCN4 uORF2 without HTS1 knockdown. Results from replicates were summed together. f, Footprint length 5′ and 3′ profile of (e). g, Rescaled counts of 5′ and 3′ ends of aligned true disome (top), sub-disome (middle), and monosome (bottom) footprints for GCN4 uORF2 after HTS1 knockdown. Results from replicates were summed together. h, Footprint length 5′ and 3′ profile of (g). i, Rescaled counts of 5′ and 3′ ends of aligned true disome (top), sub-disome (middle), and monosome (bottom) footprints for CPA1 uORF without HTS1 knockdown. Results from replicates were summed together. Both a terminating ribosome (leading) and elongating ribosome (trailing) at various codon positions are schematized. Results from replicates were summed together. j, Footprint length 5′ and 3′ profile of (i). k, Rescaled counts of 5′ and 3′ ends of aligned true disome (top), sub-disome (middle), and monosome (bottom) footprints for CPA1 uORF with HTS1 knockdown. Both an elongating ribosome stalled at the penultimate histidine codon (leading) and an elongating ribosome (trailing) are schematized. Results from replicates were summed together. l, Footprint length 5′ and 3′ profile of (k).