Extended Data Fig. 7: PSFs before and after DL-AO at various number of induced aberrations.
From: Deep learning-driven adaptive optics for single-molecule localization microscopy
(A) Examples of PSFs before and after DL-AO, when compensating artificially induced aberrations. Compensations are performed in real time during SMLM experiments shown in Extended Data Fig. 5. PSFs are measured from 100-nm-diameter crimson beads near the compensation area post SMLM acquisition. Scale bar: 5 µm. (B) PSFs are measured under instrument optimum (Methods) from 100-nm-diameter crimson beads. Scale bar: 5 µm. (C) Quantitative comparisons between PSFs measured under instrument optimum and those measured before and after DL-AO using 3D normalized cross correlation (NCC).
