Extended Data Fig. 8: Demonstrations of DL-AO correcting index mismatch induced aberration by imaging Tom20 proteins in COS-7 cells through 94 μm water-based imaging media.
From: Deep learning-driven adaptive optics for single-molecule localization microscopy
(A) 3D SMLM reconstruction of Tom20 imaged through 94 μm water-based media without AO, then reconstructed with in situ PSF model (INSPR) (B) 3D SMLM reconstruction of Tom20 imaged through 94 μm water-based media with DL-AO, then reconstructed with INSPR. (C) Axial cross-sections in A and B comparing cases without and with DL-AO combined with reconstruction methods of either in vitro PSF model (PR) or in situ PSF models (INSPR). The PR PSF model for no AO case was obtained from 100-nm-diameter crimson bead next to the imaged area. The in vitro model for DL-AO was obtained from beads at the bottom coverslip surface. Choices of in vitro model are made in order to find the closest match with corresponding experimental conditions. (D) Comparison of measured PSFs at 94 μm without and with DL-AO, in situ PSF models without and with DL-AO, and the instrument optimum. Scale bar: 2 μm.
