Extended Data Fig. 9: The reliability of locking strands in tracing molecular tension. | Nature Methods

Extended Data Fig. 9: The reliability of locking strands in tracing molecular tension.

From: Hydrogel-based molecular tension fluorescence microscopy for investigating receptor-mediated rigidity sensing

Extended Data Fig. 9

a. Sequences of 17pN-RSDTPs, paired locking strand, and unpaired locking strands. b, Representative paxillin-GFP and mTFM images of MEF cells plated on PA hydrogel with 17pN-RSDTPs in the absence of locking strands, after the addition of unpaired locking strands, and after addition of paired locking strands and c, corresponding plots of normalized accumulative intensity as a function of time (n = 6, 11, 9 cells for each condition). The images are representative of three independent experimental results. Data represent mean± s.d. of three independent experiments.

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