Extended Data Fig. 3: Calibration of RSDTPs attached to handles with different stiffness using magnetic tweezer. | Nature Methods

Extended Data Fig. 3: Calibration of RSDTPs attached to handles with different stiffness using magnetic tweezer.

From: Hydrogel-based molecular tension fluorescence microscopy for investigating receptor-mediated rigidity sensing

Extended Data Fig. 3

a, Design and sequences of handles with different stiffness. b, Illustration of calibration setup using magnetic tweezer. c, Representative force-displacement calibration curves of 17 pN (left), 45 pN (middle), and 56 pN (right) RSDTPs. d, Distribution of unfolding forces of RSDTPs attached to handles with different stiffness (400 bp, n = 51, 46, 64 events for different RSDTPs. 4000 bp, n = 45, 121, 191 events for different RSDTPs. 13571 nt, n = 39, 45, 30 events for different RSDTPs).

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