Extended Data Fig. 5: The effect of gel deformation-induced aggregation of DNA tension probes on the validity of reported signals. | Nature Methods

Extended Data Fig. 5: The effect of gel deformation-induced aggregation of DNA tension probes on the validity of reported signals.

From: Hydrogel-based molecular tension fluorescence microscopy for investigating receptor-mediated rigidity sensing

Extended Data Fig. 5: The effect of gel deformation-induced aggregation of DNA tension probes on the validity of reported signals.

a, Representative paxillin-GFP, mTFM and bright field images of cells incubated on 3 kPa PA hydrogel coated with a control RSDTP that was hardly unfolded and normal 50 pN RSDTP, and corresponding quantification of b, spreading area and c, integrin tensions signals (n = 41, 20 cells for each condition). Data represent mean± s.d. of three experiments. Two-tailed Student’s t-test is used to assess statistical significance.

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