Extended Data Fig. 9: Clustering of cytosolic G3BP1 during prolonged GEM labelling.
From: Genetically encoded multimeric tags for subcellular protein localization in cryo-EM
HeLa cells with GEM2/adaptor AAVS1 knock-in were cultured 48 h after transfection of EGFP-G3BP1 plasmid. GEM2 and adaptor expression from the AAVS1 locus was induced by 24 h doxycycline treatment. Cells were treated with rapalog for the indicated times under non-stress conditions. Formation of GEM2-EGFP-G3BP1 clusters in the cytoplasm are apparent at 4 h. Two experiments were performed with similar results.
