Extended Data Fig. 1: Phylogenetic diversity of selected encapsulins.
From: Genetically encoded multimeric tags for subcellular protein localization in cryo-EM
Maximum likelihood tree of Family 1 encapsulins as identified and constructed by Andreas et al.16, with Family 2 A encapsulin Synechococcus elongatus SrpI (GEM2) and Stenotrophomonas phage IME13 capsid protein (outgroup). Scale bar represents amino acid substitution per site. Indicated encapsulins, with GEM IDs in brackets, have been shown to form 25-nm-sized T = 1 particles, detailed in Supplementary Table 1. GEM1 has been engineered with heavy-metal-chelating elements and surface nanobodies (EMcapsulin) for room-temperature EM localization19. Also indicated is the T = 3 particle-forming encapsulin of Pyrococcus furiosus, previously used in budding yeast and HEK293 cells as a rheology probe17. Clades are shaded in alternating colours up to the most recent common ancestor between annotated sequences.
