Extended Data Fig. 5: Graphic user interface (GUI) of SBS spectral analysis.

The home-made GUI includes the parameter selection panel, 2 image panels after GPU fitting, histogram of the fitted parameter in the whole image, the raw data spectrum, the single peak fitting spectrum and the double peak fitting spectra as well as the fitted parameters at a specific pixel selected by the user. As an example, the pixel in the spinal chord central canal region at x = 9.6 µm, y = 5.2 µm position was selected and its SBG spectrum and the double-peak fitting results are shown in the GUI.