Extended Data Fig. 4: Representative 3D SBS images of a mouse fibroblast cell, human brain microvascular endothelial cells (HBMEC) and a mouse embryonic stem cell.

Three-dimensional sections of Brillouin shift (a), Brillouin linewidth (b) and Brillouin gain (c) images of a fibroblast cell under 20 mW pump and 7 mW probe with a z step of 1 µm. (d) Three-dimensional sections of Brillouin shift images of a HBMEC under 20 mW pump and 7 mW probe with a z step of 1 µm. PI, propidium iodine (viability marker that indicates damaged membranes). Brillouin shift images of HBMEC #2 (e) and HBMEC #3 (f) under 27 mW total power and 250 mW total power and the co-registered brightfield image. The overall Brillouin shift of the two cells were elevated due to high power as quantified in Fig. 2j. (g) Three-dimensional sections of Brillouin shift images of HBMEC #4 and #5 under 250 mW total power with a z step of 1 um. (h) A mouse stem cell was sequentially imaged with 27 mW pulsed and 250 mW CW schemes. Scale bar: 10 µm.