Extended Data Fig. 9: Agonist-, antagonist-, and Ca2+ ionophore-induced longitudinal changes in IMM structures revealed by fast, sustained, wide imaging.
From: StayGold variants for molecular fusion and membrane-targeting applications
HeLa cells expressing COX8a=mStayGold were continuously imaged by SDSRM (SpinSR10) at a temporal resolution of 2.5 frames per second. Two representative experimental data are shown. Histamine, cyproheptadine, and ionomycin were applied at 1 min, 2.5 min, and 4 min, respectively. The time zones of Ca2+ mobilization are shaded. IMM rearrangements were quantified by normalized cross-correlation (https://typeset.io/papers/fast-normalized-cross-correlation-1u76lu073u). Normalized cross-correlation was calculated between n and n + 1 images in individual pixels. Calculated values were averaged in each image and plotted as a function of time. Regions indicated by yellow boxes are zoomed-in for movie presentation (Supplementary Video 12). Scale bars, 10 µm. Shown are two representatives of n = 12 independent experiments (transfections) that observed histamine- and ionomycin-induced decreases in the mobility of IMM structures.
