Extended Data Fig. 10: Photostability of StayGold, mStayGold, mStayGold2, and reference green-emitting FPs in live cells under continuous WF illumination with high irradiances.
From: StayGold variants for molecular fusion and membrane-targeting applications
FPs were expressed as fusions to histone 2B (H2B) in HeLa cells and photobleached. Illumination intensities: 10, 30, 100, 300, and 1,000 W cm−2. StayGold is abbreviated as SG. a, Plot of intensity vs. normalized total exposure time, with an initial emission rate of 1,000 photons s−1 molecule−1. The curves shown are representative of three repetitions (n = 3 independent experiments). A photoactivation component was observed for both mStayGold and mStayGold2 as illumination intensity was increased. Signal fluctuation was due to some movements of intranuclear structures such as nucleoli. b, The statistical values of t1/2 (time for photobleaching from an initial emission rate of 1,000 photons s−1 molecule−1 down to 500) are plotted against the irradiances. Data points are shown as means ± s.d. (n = 3 independent experiments). The values are also shown in Supplementary Table 3. a, b, Lines are colored as follows. StayGold and td8ox2StayGold, dark green; mStayGold and mStayGoldG2, green; mEGFP, blue; mNeonGreen, red; mClover3, black; mGreenLantern, magenta.
