Extended Data Fig. 3: The evolution from (n1)StayGold to monomeric (top) and tandem dimer (bottom) variants.
From: StayGold variants for molecular fusion and membrane-targeting applications
StayGold (SG) and its variants are distinctly colored as follows. SG: khaki, oxSG: light green, ox2SG: dark sea-green, QC2-6: seafoam blue, QC2-6 FIQ (mSG): light blue. Adaptors and linkers are represented with the following deep colors. n1: black, c4: orange red, PT: purple, linker (for tandem construction): gray. #The 21-residue linker (gray) was composed of a 12-residue linker (GHGTGSTGSGSS) and a possibly protease-resistant linker2. *Although we used two linkers of 13 and 22 residues reported in the tdTomato publication to generate td6oxSG and td7oxSG, respectively, neither of them matched td5oxSG. See Supplementary Fig. 3. Except QT2-6(PT) (mSG2), all the constructs shown here are used with no tagging or for tagging the C terminus of a host protein and thus have no c4 adaptor at their C-termini; please see Supplementary Fig. 14 concerning tagging the N terminus of a host protein.
