Extended Data Fig. 3: Additional information on performance.
From: The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo

Additional information on nominal performance. (a) The spot diagram shows the distribution of rays at the imaging plane coming from a scan angle of 0°, 1°, 2°, 3°, 4°, 5°, aiming at the objective’s back aperture with a beam diameter of 20 mm, and with wavelengths of 910 nm, 920 nm, and 930 nm. (b) The simulated point spread functions from Zemax at the 5 scan angles are shown. The root-mean-square wavefront error (c) and the Strehl ration (d) as a function the scan scan angle at the back aperture is shown for the wavelength of 910 nm, 920 nm, 930 nm, and all three combined (polychromatic). The horizontal dashed line indicates that the curve below 0.072 in the RMS wavefront error plot and above 0.8 in the Strehl plot is diffraction limited. The vertical dashed line in (c) and (d) shows the nominal range of scan angle is smaller than 3 degrees. (e) The dependence of focal shift on the wavelength in the center of FOV is shown. (f) The depth of the tangential and sagittal focus over a scan angle of 5 degrees at 920 nm is shown. The horizontal dashed line shows the nominal range of the scan angle < 3°. There is only 4 µm depth difference in the center (0°) and in the edge (3°) of the FOV within the nominal scan range.