Extended Data Fig. 7: GRABDA sensors show minimal signal changes towards endogenous NE elevation.
From: Improved green and red GRAB sensors for monitoring dopaminergic activity in vivo
a, Schematic illustration (left) depicting the experimental design and representative fluorescence changes of NE2m towards systematic administration of NET blocker (3 mg/kg desipramine). b, Schematic illustration (left) depicting the experimental design and representative fluorescence changes of rDA3m towards systematic administration of NET blocker (middle) and upon optogenetic stimulations (right). c, Schematic illustration (left) depicting the experimental design and representative fluorescence changes (right) of gDA3h towards systematic administration of NET blocker (middle) and upon optogenetic stimulations (right). d, Group summary of ΔF/F0 for the indicated sensors upon opto-stimulation of VTA neurons. n = 3 mice for rDA3m and gDA3h, mean±s.e.m. Paired two-tailed Student’s t-test was performed within group. *p = 0.0167 for rDA3m and *p = 0.0463 for gDA3h. e, Group summary of ΔF/F0 for the indicated sensors towards systematic administration of NET blocker. n = 3 mice for NE2m, rDA3m and gDA3h, respectively, mean±s.e.m. Paired two-tailed Student’s t-test was performed within group. p = 0.6827, 0.2155 and 0.0012 for rDA3m, gDA3h and NE2m.
