Extended Data Fig. 8: GRABDA expression in NAc has minimal effects on DA-related animal behaviors.
From: Improved green and red GRAB sensors for monitoring dopaminergic activity in vivo
a, Schematic representation of viral injections in the bilateral NAc. b. Schematic illustration showing the open field test (OFT). c, Quantification of behavioral parameters in the OFT. n = 8, 8 and 10 mice for the control, gDA3h and rDA3h group, respectively, mean±s.e.m. One-way ANOVA was performed. p = 0.3118, 0.5870 and 0.3736. d, Schematic illustration depicting the experimental designs for panel e-f. e, Representative track of control, gDA3h and rDA3h animals. f, Quantification of behavioral parameters during the experiments. n = 8, 6 and 6 mice for the control, gDA3h and rDA3h group, respectively, mean±s.e.m. Two-tailed Student’s t tests were performed within groups: p = 0.0026, 0.0196 and 0.0039; One-way ANOVA was performed among groups: p = 0.6016. g, Schematic illustration showing the odor-reward associative learning task. h, Mean lick rate of Ctrl, gDA3h and rDA3h mice on day 1 and day 5 conditioning. n = 5 mice for each, mean±s.e.m. i, Quantification of anticipatory lick rate across five conditioning days. n = 5 mice for each, mean±s.e.m. Two-way ANOVA was performed among groups, p = 0.1076. j, Schematic diagram illustrating intravenous cocaine self-administration in rats. k, Timeline describing intravenous cocaine self-administration experiments. l, Cocaine infusions over 10 days of SA training did not differ between rats expressing gDA3m and eGFP virus bilaterally in the NAc core. n = 8 rats for each, mean±s.e.m. Two-way ANOVA mixed-effects model (Day x Virus): Day, F(9,126) = 4.50, p = 0.00004; Virus, F(1,14) = 0.35, p = 0.56; Day x Virus, F(9,126) = 0.21, p = 0.99. m, Nose-pokes in the active and inactive ports over the last 3 days of SA training did not differ between virus groups. Two-way ANOVA mixed-effects models (Day x Virus). n = 8 rats for each, mean±s.e.m. Active port: Day, F(2,28) = 3.21, p = 0.06; Virus, F(1,14) = 1.35, p = 0.27; Day x Virus, F(2,28) = 1.48, p = 0.24. Inactive port: Day, F(2, 28) = 1.97, p = 0.16; Virus, F(1,14) = 0.48, p = 0.50; Day x Virus, F(2,28) = 0.92, p = 0.41.
