Extended Data Fig. 3: Kinetics measurement of GRABDA sensors in HEK293T cells.
From: Improved green and red GRAB sensors for monitoring dopaminergic activity in vivo
a, Schematic illustration showing the local perfusion system using a glass pipette containing 100 μM DA and/or receptor-specific antagonist positioned above the sensor-expressing cell. The yellow line indicates the area for line scanning. The dash lines indicate the pipette. Scale bar, 20 μm. b, Representative traces showing the response measured using line-scanning; when indicated, DA and receptor-specific antagonist were puffed onto the cell. The trace were the average of 3 different ROIs on the scanning line. Data are shown as mean ± SD. Each trace was fitted with a single-exponential function to determine the τon (left) and τoff (right). c, Group summary of τon and τoff. τon, n = 11, 8, 11, 6, 9, 8 cells for gDA3m, gDA3h, rDA2m, rDA2h, rDA3m, rDA3h; τoff, n = 10, 14, 9, 7, 10, 6 cells for gDA3m, gDA3h, rDA2m, rDA2h, rDA3m, rDA3h, mean±s.e.m.
