Extended Data Fig. 5: Colocalization analysis of SynapShot signals with endogenous synaptic markers.
From: Real-time visualization of structural dynamics of synapses in live cells in vivo
a, Representative confocal images and pie chart showing co-localization of SynapShot and Bassoon as a synaptic marker detected by immunofluorescence. Scale bar, 5 μm. Percentage of co-localization of Bassoon with SynapShot signals (right; Bassoon total n = 443). b, Confocal images and pei charts showing co-localization of SynapShot with vGLUT or Gephyrin in cultured hippocampal neurons. Scale bar, 5 μm. Percentage of co-localization of vGLUT or Gephyrin with SynapShot signals (right; vGLUT1 total n = 440, Gephyrin total n = 623). b, Representative confocal images showing mGRASP-based SynapShot signals with immunostained synaptic markers and pie charts summarizing proportion of punctum clusters of SynapShot with synaptic markers. (Total number for Homer1 n = 1624, Synapsin1 n = 1767, Bassoon n = 1637, Gephyrin n = 781.) Scale bar, 5 μm.
