Extended Data Fig. 7: Measurement of the exchange of reporter molecules in ddFP or split-FP signals in a single synapse. | Nature Methods

Extended Data Fig. 7: Measurement of the exchange of reporter molecules in ddFP or split-FP signals in a single synapse.

From: Real-time visualization of structural dynamics of synapses in live cells in vivo

Extended Data Fig. 7

a, Time-lapse images showing the ddFP (top) or split-FP (bottom) signal changes after photobleaching in cultured hippocampal neurons. Scale bar, 5 μm. b, Graph showing recovery curves after photobleaching of each green signal in synapses (ddGFP, n = 16 puncta; split-GFP, n = 18 puncta).

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