Extended Data Fig. 4: Effects of the expression level of proteins on the SynapShot signals in cultured hippocampal neurons.
From: Real-time visualization of structural dynamics of synapses in live cells in vivo
a, Confocal images showing expression of Synapshot constructs and ddGFP signals carried by CAG promoter(left) or CamKIIa(right) in cultured hippocampal neurons. Scale bar, 5 μm. b-c, Bar graph showing size (b) and mean intensity (c) of the SynapShot signal. Data represent the mean ± s.e.m. (CAG, n = 32 puncta; CamKIIa, n = 70 puncta; n.s., not significant; **** p < 0.0001, Student’s two-tailed t-test).
