Extended Data Fig. 5: Direct comparison shows that the mEnrich-seq design has better fold enrichment than REMoDE.
From: mEnrich-seq: methylation-guided enrichment sequencing of bacterial taxa of interest from microbiome
a, Agarose gel electrophoresis of Mock-3 (a mock with five species, see Supplementary Information) after treatment by RE and T5 exonuclease for once. Left, Agarose gel electrophoresis of the mock sample digested by RE, XapI. Due to the sparse RE recognition sites in the background genomes in the mock microbiome sample, relatively long DNA fragments from the background genomes remain even after RE digestion; Right, The gel image of the mock sample digested by RE and T5. b, A direct comparison between mEnrich-seq and REMoDE was performed using the same mock microbiome sample (Mock-3), and the same sequencing platform (Illumina). To be compatible with the short-read sequencing platform, the library of mEnrich-seq was prepared according to the ‘Compatibility between mEnrich-seq and other sequencing platforms’ section in the Supplementary Information.
