Extended Data Fig. 2: Effect of phosphopantetheinyl transferase NpgA on luminescence in plant cells.
From: An improved pathway for autonomous bioluminescence imaging in eukaryotes
a. Transient expression in N. benthamiana leaves (N = 9 leaves per box plot). b. Transient expression in P. hybrida flowers (N = 13 and N = 15 leaves for FBP1 + NpgA and FBP1, respectively). c. Transient expression in BY-2 cells (N = 14 plant cell packs per box plot). In these experiments, pX037 plasmid was co-infiltrated with a plasmid encoding NpgA. The boxes are the first and the third quartiles, whiskers are the rest of the distribution except outliers, the orange line is the median. The difference between mean values and p-values of post-hoc two-sided Mann-Whitney U-tests are indicated below the brackets between the box plots, p = 7.9e-04 (a), p = 8.0e-06 (b), p = 7.0e-06 (c). In these experiments, each gene was delivered on a separate plasmid.
