Extended Data Fig. 8: ZymoBIOMICS samples contain premature lysis and under-representation of gram-negative bacteria in single-cell sequencing.
From: Massively parallel single-cell sequencing of diverse microbial populations
This chart shows DoTA-seq relative abundance of a Zymobiomics microbial community standard, which is a commercial mock microbial community mix preserved in DNA/RNA shield. Comparing the relative abundances with and without washing the cells prior to droplet making, we found that Gram-negative cells (in red) are almost undetected after 2X wash. This suggests that gram-negative cells were already lysed in the buffer and washing removed the cell debris and free-floating genomic DNA, leaving only the gram-positive cells intact for single-cell sequencing.
