Extended Data Fig. 5: Comparison of the image quality using different light-sheet microscopes.

a) Cross section in XZ plane of the intestinal organoid shown in Fig. 1g using Detection 1, Detection 2 and the fused data from both objectives. Scale bar 50 µm. b) Comparison of the image quality using the Shannon Entropy of the Discrete Cosine Transform (DCT) as metric for Detection 1, Detection 2 and the fused data. The DCT is calculated for each z section of the image stack corresponding to a. c) Cross section in XZ plane of a human colon cancer organoid using Detection 1, Detection 2 and the fused data from both objectives. The experiment was repeated two times with 7 and 8 organoids imaged. Scale bar 50 µm. d) Comparison of the image quality using the Shannon Entropy of the DCT as metric for Detection 1, Detection 2 and the fused data. The DCT is calculated for each z section of the image stack corresponding to c. e) Maximum intensity projection (MIP) along the Z axis and a cross section in the XZ plane of a gastruloid stained with the nuclear marker DRAQ5 imaged with the light-sheet microscope published in¹⁷. In total n = 9 gastruloids were imaged. Scale bar 50 µm. f) Image quality plot using Shannon Entropy of the DCT along individual z sections of the image stack corresponding to e. g) Maximum intensity projection (MIP) along the Z axis and a cross section in the XZ plane of a gastruloid stained with the nuclear marker DRAQ5 imaged with the light-sheet microscope presented in this work. In total n = 7 gastruloids were imaged. Scale bar 50 µm. h) Image quality plot using Shannon Entropy of the DCT along individual z sections of the image stack corresponding to g.