Extended Data Fig. 3: Comparison of Metabuli to best performing AA- and DNA-based tools on real long-read metagenomic samples.
From: Metabuli: sensitive and specific metagenomic classification via joint analysis of amino acid and DNA
In contrast to Fig. 2g–h, Kraken2X instead of Kaiju is utilized due to its superior performance on long reads. The databases were built using GTDB genomes and a human genome (T2T-CHM13v2.0) based on GTDB taxonomy edited to include a human taxon. Real nanopore sequencing data from human gut (a) and marine (b) environments, as well as PacBio HiFi reads from human gut (c) and marine (d) environments, were classified by each tool. The area is proportional to the number of reads within each panel. The proportion of reads classified by each tool is denoted in parentheses.
